We evaluate zebrafish as a potential animal model for studies of the genetic cellular and signaling basis of emmetropization and myopia. Heartbeat measurement is important in assesssing cardiac function because variations in heart rhythm can be the cause as well as an effect of hidden pathological heart conditions. 1a shows a schematic drawing of the zuve technique. Therefore organs including the heart can be observed and analyzed in a non invasive manner. Refractive errors in vision can be caused by aberrant axial length of the eye irregular corneal shape or lens abnormalities. Zebrafish were anesthetized with tricaine methanesulfonate ms222 at a dose of 040 gkg in 0075 gl instant ocean solution hu et al 2000 and transferred to a shallow concave trough of a custombuilt glass chamber.
Using data acquired from 160 larvae the half life of a zebrafish tissue neutrophil during the 48 hour measurement window was found to be in the region of 120 hours 1177 hrs 95 confidence interval 95671578. 4 consistency in handling the fish positioning of the fish time of anesthesia and duration between anesthesia and measurement is crucial for consistent results. The measurement of blood glucose level is a commonly used method for assessing beta cell function but the small size of the zebrafish presents challenges both for collecting blood samples and for. A zebrafish was considered anesthetized when its respiration rate decreased as visually noticead by its gill movement. Previously heart rate measurement was analyzed by a complicated system programming language fluorescence transgenic zebrafish or expensive setup that was not feasible and practical. Methods for zebrafish pancreas function have been established including fasting and postprandial glucose measurement and intraperitoneal glucose tolerance tests as well as techniques for pancreas dissection and islet cell culture eames et al 2010.
Zebrafish has an advantage of optical clarity within the body during its early developmental stage. The zebrafish has become increasingly useful as a model system for understanding the molecular basis of movement and behaviour. Automated measurement of zebrafish movement using this technique will be useful for multiple applications in neuroscience pharmacology and neuropsychiatry. Axial length and other eye dimensions of. 5 we anesthetize 46 week zebrafish for 2 min and 16 week zebrafish for 25 min in 1 tricaine and image the heart by 3 or 35 min respectively. Eames nalle et al 2017.
Causes of eye length overgrowth include multiple genetic loci and visual parameters. Three minutes of anesthesia was designed for the zebrafish study because the zebrafish experimental setup and tests could be finished within three minutes.
Gallery of Zebrafish Measurement
